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Citation
Lee, J.R., Urban, S., Garvey, C.F., Freeman, M. (2001). Regulated intracellular ligand transport and proteolysis control EGF signal activation in Drosophila.  Cell 107(2): 161--171.
FlyBase ID
FBrf0139606
Publication Type
Research paper
Abstract

The membrane proteins Star and Rhomboid-1 have been genetically defined as the primary regulators of EGF receptor activation in Drosophila, but their molecular mechanisms have been elusive. Both Star and Rhomboid-1 have been assumed to work at the cell surface to control ligand activation. Here, we demonstrate that they control receptor signaling by regulating intracellular trafficking and proteolysis of the ligand Spitz. Star is present throughout the secretory pathway and is required to export Spitz from the endoplasmic reticulum to the Golgi apparatus. Rhomboid-1 is localized in the Golgi, where it promotes the cleavage of Spitz. This defines a novel growth factor release mechanism that is distinct from metalloprotease-dependent shedding from the cell surface.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference
    Alleles (12)
    Gene Groups (1)
    Genes (7)
    Physical Interactions (3)
    Cell Lines (1)
    Insertions (2)
    Experimental Tools (4)
    Transgenic Constructs (8)