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Ryoo, H.D., Bergmann, A., Gonen, H., Ciechanover, A., Steller, H. (2002). Regulation of Drosophila IAP1 degradation and apoptosis by reaper and ubcD1.  Nat. Cell Biol. 4(6): 432--438.
FlyBase ID
FBrf0149132
Publication Type
Research paper
Abstract

Cell death in higher organisms is negatively regulated by Inhibitor of Apoptosis Proteins (IAPs), which contain a ubiquitin ligase motif, but how ubiquitin-mediated protein degradation is regulated during apoptosis is poorly understood. Here, we report that Drosophila melanogaster IAP1 (DIAP1) auto-ubiquitination and degradation is actively regulated by Reaper (Rpr) and UBCD1. We show that Rpr, but not Hid (head involution defective), promotes significant DIAP1 degradation. Rpr-mediated DIAP1 degradation requires an intact DIAP1 RING domain. Among the mutations affecting ubiquitination, we found ubcD1, which suppresses rpr-induced apoptosis. UBCD1 and Rpr specifically bind to DIAP1 and stimulate DIAP1 auto-ubiquitination in vitro. Our results identify a novel function of Rpr in stimulating DIAP1 auto-ubiquitination through UBCD1, thereby promoting its degradation.

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Erratum

Erratum: Regulation of Drosophila IAP1 degradation and apoptosis by reaper and ubcD1.
Ryoo et al., 2002, Nat. Cell Biol. 4(7): 546 [FBrf0161511]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Cell Biol.
    Title
    Nature Cell Biology
    Publication Year
    1999-
    ISBN/ISSN
    1465-7392 1476-4679
    Data From Reference