Open Close
Reference
Citation
Chen, E.H., Pryce, B.A., Tzeng, J.A., Gonzalez, G.A., Olson, E.N. (2003). Control of myoblast fusion by a guanine nucleotide exchange factor, loner, and its effector ARF6.  Cell 114(6): 751--762.
FlyBase ID
FBrf0161992
Publication Type
Research paper
Abstract

Myoblast fusion is essential for the formation and regeneration of skeletal muscle. In a genetic screen for regulators of muscle development in Drosophila, we discovered a gene encoding a guanine nucleotide exchange factor, called loner, which is required for myoblast fusion. Loner localizes to subcellular sites of fusion and acts downstream of cell surface fusion receptors by recruiting the small GTPase ARF6 and stimulating guanine nucleotide exchange. Accordingly, a dominant-negative ARF6 disrupts myoblast fusion in Drosophila embryos and in mammalian myoblasts in culture, mimicking the fusion defects caused by loss of Loner. Loner and ARF6, which also control the proper membrane localization of another small GTPase, Rac, are key components of a cellular apparatus required for myoblast fusion and muscle development. In muscle cells, this fusigenic mechanism is coupled to fusion receptors; in other fusion-competent cell types it may be triggered by different upstream signals.

PubMed ID
PubMed Central ID
Related Publication(s)
Personal communication to FlyBase

MHC-tauGFP construct.
Chen, 2004.3.1, MHC-tauGFP construct. [FBrf0178739]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference