Subject: Akt1 constructs and insertions
The following information was provided by Annette Parks of Exelixis, Inc.
In P{UAS-Akt1.Exel}, wild type sequences for the short, 60 kD form of Akt1
(GenBank accession X83510) as described in Andjelkovic et al., 1995 (J.
Biol. Chem. 270: 4066-4075; FBrf0079853) were cloned into P{Express-UAS}.
P{GMR-Akt1.Exel}consists of the same sequence cloned into P{Express-glass}.
P{GMR-Akt1.T342D.S505D}consists of the same sequence with Thr342Asp and
Ser505Asp mutations cloned into P{Express-glass}. The protein variant is
constitutively active.
P{UAS-Akt1.Exel}1, P{GMR-Akt1.T342D.S505D}1 and P{GMR-Akt1.Exel}1 are
homozygous and hemizygous viable and fertile, X chromosome insertions.
P{UAS-Akt1.Exel}2, P{GMR-Akt1.Exel}2 and P{GMR-Akt1.T342D.S505D}2 are
homozygous viable and fertile, second chromosome insertions.
P{GMR-Akt1.T342D.S505D}3 is a homozygous viable and fertile, third
chromosome insertion.