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Citation
Lin, J.M., Schroeder, A., Allada, R. (2005). In vivo circadian function of casein kinase 2 phosphorylation sites in Drosophila PERIOD.  J. Neurosci. 25(48): 11175--11183.
FlyBase ID
FBrf0191049
Publication Type
Research paper
Abstract

Phosphorylation plays a key role in the precise timing of circadian clocks. Daily rhythms of phosphorylation of the Drosophila circadian clock component PERIOD (PER) were first described more than a decade ago, yet little is known about their phosphorylation sites and their function in circadian behavior. Here we show that serines 151 and 153 in PER are required for robust in vitro phosphorylation by the casein kinase 2 (CK2) holoenzyme, a cytoplasmic kinase shown to be involved in circadian rhythms. Mutation of these sites in transgenic flies results in significant period lengthening of behavioral rhythms, altered PER rhythms, and delayed PER nuclear localization in circadian pacemaker neurons. In many respects, mutation of these phosphorylation sites phenocopies mutation of the catalytic subunit of CK2. We propose that CK2 phosphorylation at these sites triggers PER nuclear localization.

PubMed ID
PubMed Central ID
PMC6725653 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Neurosci.
    Title
    Journal of Neuroscience
    Publication Year
    1981-
    ISBN/ISSN
    0270-6474 1529-2401
    Data From Reference
    Alleles (14)
    Genes (4)
    Natural transposons (1)
    Experimental Tools (1)
    Transgenic Constructs (10)