Open Close
Dorus, S., Busby, S.A., Gerike, U., Shabanowitz, J., Hunt, D.F., Karr, T.L. (2006). Genomic and functional evolution of the Drosophila melanogaster sperm proteome.  Nat. Genet. 38(12): 1440--1445.
FlyBase ID
Publication Type
Research paper

In addition to delivering a haploid genome to the egg, sperm have additional critical functions, including egg activation, origination of the zygote centrosome and delivery of paternal factors. Despite this, existing knowledge of the molecular basis of sperm form and function is limited. We used whole-sperm mass spectrometry to identify 381 proteins of the Drosophila melanogaster sperm proteome (DmSP). This approach identified mitochondrial, metabolic and cytoskeletal proteins, in addition to several new functional categories. We also observed nonrandom genomic clustering of sperm genes and underrepresentation on the X chromosome. Identification of widespread functional constraint on the proteome indicates that sexual selection has had a limited role in the overall evolution of D. melanogaster sperm. The relevance of the DmSP to the study of mammalian sperm function and fertilization mechanisms is demonstrated by the identification of substantial homology between the DmSP and proteins of the mouse axoneme accessory structure.

PubMed ID
PubMed Central ID
Related Publication(s)
Personal communication to FlyBase

Resolution of Dorus et al. (2006) gene mismatches.
Dorus, 2008.10.28, Resolution of Dorus et al. (2006) gene mismatches. [FBrf0206128]

Associated Information
Associated Files
Other Information
Secondary IDs
    Language of Publication
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Nat. Genet.
    Nature Genetics
    Publication Year
    1061-4036 1546-1718
    Data From Reference