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Citation
Glater, E.E., Megeath, L.J., Stowers, R.S., Schwarz, T.L. (2006). Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent.  J. Cell Biol. 173(4): 545--557.
FlyBase ID
FBrf0194133
Publication Type
Research paper
Abstract

Mitochondria are distributed within cells to match local energy demands. We report that the microtubule-dependent transport of mitochondria depends on the ability of milton to act as an adaptor protein that can recruit the heavy chain of conventional kinesin-1 (kinesin heavy chain [KHC]) to mitochondria. Biochemical and genetic evidence demonstrate that kinesin recruitment and mitochondrial transport are independent of kinesin light chain (KLC); KLC antagonizes milton's association with KHC and is absent from milton-KHC complexes, and mitochondria are present in klc (-/-) photoreceptor axons. The recruitment of KHC to mitochondria is, in part, determined by the NH(2) terminus-splicing variant of milton. A direct interaction occurs between milton and miro, which is a mitochondrial Rho-like GTPase, and this interaction can influence the recruitment of milton to mitochondria. Thus, milton and miro are likely to form an essential protein complex that links KHC to mitochondria for light chain-independent, anterograde transport of mitochondria.

PubMed ID
PubMed Central ID
PMC2063864 (PMC) (EuropePMC)
Related Publication(s)
Note

Paradigm lost: milton connects kinesin heavy chain to miro on mitochondria.
Rice and Gelfand, 2006, J. Cell Biol. 173(4): 459--461 [FBrf0194320]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference
    Alleles (4)
    Genes (6)
    Physical Interactions (8)
    Transgenic Constructs (2)