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Christensen, S., Cook, K. (2007.3.22). Isolation and characterization of Df(2R)BSC270. 
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Date: Thu, 22 Mar 2007  14:52:27  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2R)BSC270
Cc: Stacey Christensen <sjchristXXXX>, mdealXXXX, kaufman@XXXX
Isolation and characterization of Df(2R)BSC270
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC270 was isolated as a FLP recombinase-induced recombination 
event involving PBac{RB}e01587 and P{XP}d01074. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; PBac{RB}e01587/P{XP}d01074 males crossed to 
w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.RB3}BSC270 from the segment of 
PBac{RB}e01587 to the left of its FRT site and the segment of 
P{XP}d01074 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. The 
cytological breakpoints of Df(2R)BSC270 predicted from the Release 4 
genomic coordinates of the transposable element insertions sites are 
44F9;45A2. It failed to complement Df(2R)ED1791 and Df(2R)ED1770.
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    Aberrations (3)
    Insertions (3)
    Transgenic Constructs (1)