Open Close
Reference
Citation
Christensen, S., Cook, K. (2007.3.22). Isolation and characterization of Df(2R)BSC274. 
FlyBase ID
FBrf0199297
Publication Type
Personal communication to FlyBase
Abstract
PubMed ID
PubMed Central ID
Text of Personal Communication
Date: Thu, 22 Mar 2007  14:52:32  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2R)BSC274
Isolation and characterization of Df(2R)BSC274
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC274 was isolated as a FLP recombinase-induced recombination 
event involving PBac{RB}CG17034[e02894] and P{XP}CG6191[d03529]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of P{hsFLP}1, y[1] w[1118]; 
PBac{RB}CG17034[e02894]/P{XP}CG6191[d03529] males crossed to w[1118]; 
P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as 
larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.RB3}BSC274 from the segment of 
PBac{RB}CG17034[e02894] to the left of its FRT site and the segment 
of P{XP}CG6191[d03529] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' 
for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in 
the Supplementary Methods. The cytological breakpoints of 
Df(2R)BSC274 predicted from the Release 4 genomic coordinates of the 
transposable element insertions sites are 50A7;50B4. It failed to 
complement cnn[HK21] and EfTuM[L4569].
DOI
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Abbreviation
    Title
    ISBN/ISSN
    Data From Reference
    Aberrations (1)
    Alleles (2)
    Genes (2)
    Insertions (3)
    Transgenic Constructs (1)