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Christensen, S., Cook, K. (2007.5.8). Isolation and characterization of Df(2R)BSC306. 
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From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	mdealXXXX, Stacey Christensen <sjchristXXXX>, kaufman@XXXX
Subject: 	Isolation and characterization of Df(2R)BSC306
Date: 	Tue, 08 May 2007  08:46:36  -0400  ( 13:46  BST)
Isolation and characterization of Df(2R)BSC306
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC306 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f04713 and P{XP}CG6145[d02819]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; PBac{WH}f04713/P{XP}CG6145[d02819] males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC306 from the segment 
of PBac{WH}f04713 to the left of its FRT site and the segment of 
P{XP}CG6145[d02819] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(2R)BSC306 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 50A3;50B1. It failed to complement cnn[HK21] and EfTuM[L4569].
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    Aberrations (1)
    Alleles (2)
    Genes (2)
    Insertions (3)
    Transgenic Constructs (1)