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Christensen, S., Gresens, J., Cook, K., Cook, K. (2007.10.29). Isolation and characterization of Df(3R)BSC320. 
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FBrf0200316
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To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3R)BSC320
Date: Mon, 29 Oct 2007  12:07:16  -0400
Df(3R)BSC320 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}cas[d10725] and PBac{WH}f00649. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; P{XP}cas[d10725]/PBac{WH}f00649 males. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC320 from the segment of 
P{XP}cas[d10725] to the left of its FRT site and the segment of 
PBac{WH}f00649 to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(3R)BSC320 predicted from the 
Release 5 genomic coordinates of the transposable element insertion 
sites are 83C1;83D4. Df(3R)BSC320 failed to complement Rm62[EY06795] 
and Df(3R)Exel7284. Df(3R)FDD-0116468 is a synonym for Df(3R)BSC320.
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    English
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    Aberrations (2)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)