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Citation
Pal, S., Leger, R.J.S., Wu, L.P. (2007). Fungal peptide destruxin a plays a specific role in suppressing the innate immune response in Drosophila melanogaster.  J. Biol. Chem. 282(12): 8969--8977.
FlyBase ID
FBrf0200357
Publication Type
Research paper
Abstract

Destruxins are a class of insecticidal, anti-viral, and phytotoxic cyclic depsipeptides that are also studied for their toxicity to cancer cells. They are produced by various fungi, and a direct relationship has been established between Destruxin production and the virulence of the entomopathogen Metarhizium anisopliae. Aside from opening calcium channels, their in vivo mode of action during pathogenesis remains largely uncharacterized. To better understand the effects of a Destruxin, we looked at changes in gene expression following injection of Destruxin A into the fruit fly Drosophila melanogaster. Microarray results revealed reduced expression of various antimicrobial peptides that play a major role in the humoral immune response of the fly. Flies co-injected with a non-lethal dose of Destruxin A and the normally innocuous Gram-negative bacteria Escherichia coli, showed increased mortality and an accompanying increase in bacterial titers. Mortality due to sepsis was rescued through ectopic activation of components in the IMD pathway, one of two signal transduction pathways that are responsible for antimicrobial peptide induction. These results demonstrate a novel role for Destruxin A in specific suppression of the humoral immune response in insects.

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PubMed Central ID
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Secondary IDs
  • FBrf0193030
Language of Publication
English
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Parent Publication
Publication Type
Journal
Abbreviation
J. Biol. Chem.
Title
Journal of Biological Chemistry
Publication Year
1905-
ISBN/ISSN
0021-9258
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