Open Close
Reference
Citation
Eulalio, A., Huntzinger, E., Izaurralde, E. (2008). GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay.  Nat. Struct. Mol. Biol. 15(4): 346--353.
FlyBase ID
FBrf0204460
Publication Type
Research paper
Abstract

MicroRNAs (miRNAs) silence gene expression by binding 3' untranslated regions of target mRNAs. Recent studies suggested silencing is achieved through either recruitment of eIF6, which prevents ribosome assembly, or displacement of eIF4E from the mRNA 5' cap structure. Using Drosophila melanogaster cells, we show that eIF6 is not required for silencing. In contrast, silencing is abolished by mutating Argonaute 1 (AGO1) at two conserved phenylalanine residues predicted to mediate binding to the cap structure. Notably, we found these mutations also prevented AGO1 from interacting with GW182 and miRNAs, indicating that the essential role of these residues is unrelated to cap binding. Consistently, depleting GW182 or overexpressing its AGO1 binding domain relieved silencing of all reporters tested, including those lacking a poly(A) tail. Together, our findings show that miRNA function is effected by AGO1-GW182 complexes and the role of GW182 in silencing goes beyond promoting deadenylation.

PubMed ID
PubMed Central ID
Related Publication(s)
Note

Think you know how miRNAs work? Think again.
Richter, 2008, Nat. Struct. Mol. Biol. 15(4): 334--336 [FBrf0216028]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Struct. Mol. Biol.
    Title
    Nature Structural and Molecular Biology
    Publication Year
    2004-
    ISBN/ISSN
    1545-9993 1545-9985
    Data From Reference