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Christensen, S., Cook, K., Cook, K. (2008.4.15). Isolation and characterization of Df(3L)BSC440. 
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From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(3L)BSC440
Date: 	Tue, 15 Apr 2008  14:36:16  -0400  ( 19:36  BST)
Isolation and characterization of Df(3L)BSC440
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC440 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d06287 and PBac{WH}f03465. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; P{XP}d06287/PBac{WH}f03465 males. The males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC440 from the segment of P{XP}d06287 
to the left of its FRT site and the segment of PBac{WH}f03465 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. Exelixis, Inc. 
determined the insertion site of P{XP}d06287 to be Release 3 genomic 
coordinate 688843 on chromosome arm 3L and the insertion site of 
PBac{WH}f03465 to be Release 3 genomic coordinate 807399 on arm 3L. 
The Gene Disruption project determined the insertion site of 
P{XP}d06287 to be Release 3 genomic coordinate 688835 on arm 3L. The 
P{XP}d06287 insertion site corresponds to 61C8 and the PBac{WH}f03465 
insertion site corresponds to 61D2 on the Release 3 and Release 5 
genome maps. Consequently, the cytological breakpoints of 
Df(3L)BSC440 are predicted to be 61C8;61D2. Df(3L)BSC440 failed to 
complement emc[1] and Df(3L)ED202.
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    Aberrations (2)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (2)