Isolation and characterization of Df(3L)BSC440
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC440 was isolated as a FLP recombinase-induced recombination event involving P{XP}d06287 and PBac{WH}f03465. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d06287/PBac{WH}f03465 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC440 from the segment of P{XP}d06287 to the left of its FRT site and the segment of PBac{WH}f03465 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d06287 to be Release 3 genomic coordinate 688843 on chromosome arm 3L and the insertion site of PBac{WH}f03465 to be Release 3 genomic coordinate 807399 on arm 3L. The Gene Disruption project determined the insertion site of P{XP}d06287 to be Release 3 genomic coordinate 688835 on arm 3L. The P{XP}d06287 insertion site corresponds to 61C8 and the PBac{WH}f03465 insertion site corresponds to 61D2 on the Release 3 and Release 5 genome maps. Consequently, the cytological breakpoints of Df(3L)BSC440 are predicted to be 61C8;61D2. Df(3L)BSC440 failed to complement emc1 and Df(3L)ED202.