Open Close
Reference
Citation
Christensen, S., Cook, K., Cook, K. (2008.4.15). Isolation and characterization of Df(3L)BSC440. 
FlyBase ID
FBrf0204475
Publication Type
Personal communication to FlyBase
Abstract
PubMed ID
PubMed Central ID
Text of Personal Communication
From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(3L)BSC440
Date: 	Tue, 15 Apr 2008  14:36:16  -0400  ( 19:36  BST)
Isolation and characterization of Df(3L)BSC440
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC440 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d06287 and PBac{WH}f03465. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; P{XP}d06287/PBac{WH}f03465 males. The males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC440 from the segment of P{XP}d06287 
to the left of its FRT site and the segment of PBac{WH}f03465 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. Exelixis, Inc. 
determined the insertion site of P{XP}d06287 to be Release 3 genomic 
coordinate 688843 on chromosome arm 3L and the insertion site of 
PBac{WH}f03465 to be Release 3 genomic coordinate 807399 on arm 3L. 
The Gene Disruption project determined the insertion site of 
P{XP}d06287 to be Release 3 genomic coordinate 688835 on arm 3L. The 
P{XP}d06287 insertion site corresponds to 61C8 and the PBac{WH}f03465 
insertion site corresponds to 61D2 on the Release 3 and Release 5 
genome maps. Consequently, the cytological breakpoints of 
Df(3L)BSC440 are predicted to be 61C8;61D2. Df(3L)BSC440 failed to 
complement emc[1] and Df(3L)ED202.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
DOI
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Abbreviation
    Title
    ISBN/ISSN
    Data From Reference
    Aberrations (2)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (2)