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Christensen, S., Cook, K., Cook, K. (2008.4.15). Isolation and characterization of Df(3L)BSC418 
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From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(3L)BSC418
Date: 	Tue, 15 Apr 2008  14:21:36  -0400  ( 19:21  BST)
Isolation and characterization of Df(3L)BSC418
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC418 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d09186 and PBac{WH}CG32444[f00963]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; P{XP}d09186/PBac{WH}CG32444[f00963] males. The males were 
heat shocked as larvae as described in Parks et al., Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.WH5}BSC418 from the segment of 
P{XP}d09186 to the left of its FRT site and the segment of 
PBac{WH}CG32444[f00963] to the right of its FRT site. Its presence 
was verified using the PCR methods and primers described in Parks et 
al. The cytological breakpoints of Df(3L)BSC418 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 78C9;78E1. Df(3L)BSC418 failed to complement croc[5F59] and Hr78[2].
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    Aberrations (1)
    Alleles (2)
    Genes (2)
    Insertions (3)
    Transgenic Constructs (1)