Isolation and characterization of Df(3L)BSC418
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC418 was isolated as a FLP recombinase-induced recombination event involving P{XP}d09186 and PBac{WH}CG32444f00963. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d09186/PBac{WH}CG32444f00963 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC418 from the segment of P{XP}d09186 to the left of its FRT site and the segment of PBac{WH}CG32444f00963 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC418 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 78C9;78E1. Df(3L)BSC418 failed to complement croc5F59 and Hr782.