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Christensen, S., Cook, K., Cook, K. (2008.4.15). Isolation and characterization of Df(3R)BSC486. 
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From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Subject: 	Isolation and characterization of Df(3R)BSC486
Date: 	Tue, 15 Apr 2008  15:07:13  -0400  ( 20:07  BST)
Isolation and characterization of Df(3R)BSC486
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC486 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}CG5245[f05954] and P{XP}d04743. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; PBac{WH}CG5245[f05954]/P{XP}d04743 males. The males were 
heat shocked as larvae as described in Parks et al., Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.WH5}BSC486 from the segment of 
PBac{WH}CG5245[f05954] to the left of its FRT site and the segment of 
P{XP}d04743 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. The 
cytological breakpoints of Df(3R)BSC486 predicted from the Release 5 
genomic coordinates of the transposable element insertions sites are 
87B10;87E9. Df(3R)BSC486 failed to complement Vha55[16].
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    Aberrations (1)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)