Isolation and characterization of Df(3L)BSC575
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC575 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG7304f05948 and P{XP}brmd00415. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG7304f05948/P{XP}brmd00415 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC575 from the segment of PBac{WH}CG7304f05948 to the left of its FRT site and the segment of P{XP}brmd00415 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC575 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 71F1;72C1. Df(3L)BSC575 failed to complement Df(3L)BSC443 and brm2.