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Christensen, S., Cook, K., Cook, K. (2008.7.11). Isolation and characterization of Df(3L)BSC575. 
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From: 	Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC575
Date: 	Fri, 11 Jul 2008  13:23:38  -0400  ( 18:23  BST)
Isolation and characterization of Df(3L)BSC575
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC575 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}CG7304[f05948] and P{XP}brm[d00415]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] females crossed to 
P{hsFLP}1, y[1] w[1118]; PBac{WH}CG7304[f05948]/P{XP}brm[d00415] 
males. The males were heat shocked as larvae as described in Parks et 
al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and 
crosses in preceding and succeeding generations maintained the 
original genetic background of the Exelixis insertion stocks 
(Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). 
The recombination event generated the genetic element 
P+PBac{XP5.WH5}BSC575 from the segment of PBac{WH}CG7304[f05948] to 
the left of its FRT site and the segment of P{XP}brm[d00415] to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. The cytological 
breakpoints of Df(3L)BSC575 predicted from the Release 5 genomic 
coordinates of the transposable element insertions sites are 
71F1;72C1. Df(3L)BSC575 failed to complement Df(3L)BSC443 and brm[2].
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    Aberrations (2)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)