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Reference
Citation
Bereczki, O., Ujfaludi, Z., Pardi, N., Nagy, Z., Tora, L., Boros, I.M., Balint, E. (2008). TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.  BMC Mol. Biol. 9(): 57.
FlyBase ID
FBrf0205137
Publication Type
Research paper
Abstract

The tumour suppressor protein p53 is a sequence specific DNA-binding transcription regulator, which exerts its versatile roles in genome protection and apoptosis by affecting the expression of a large number of genes. In an attempt to obtain a better understanding of the mechanisms by which p53 transcription function is regulated, we studied p53 interactions.We identified BIP2 (Bric-à-brac interacting protein 2), the fly homolog of TAF3, a histone fold and a plant homeodomain containing subunit of TFIID, as an interacting partner of Drosophila melanogaster p53 (Dmp53). We detected physical interaction between the C terminus of Dmp53 and the central region of TAF3 both in yeast two hybrid assays and in vitro. Interestingly, DmTAF3 can also interact with human p53, and mammalian TAF3 can bind to both Dmp53 and human p53. This evolutionarily conserved interaction is functionally significant, since elevated TAF3 expression severely and selectively inhibits transcription activation by p53 in human cell lines, and it decreases the level of the p53 protein as well.We identified TAF3 as an evolutionarily conserved negative regulator of p53 transcription activation function.

PubMed ID
PubMed Central ID
PMC2441632 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    BMC Mol. Biol.
    Title
    BMC Molecular Biology
    Publication Year
    2000-
    ISBN/ISSN
    1471-2199
    Data From Reference
    Genes (2)
    Physical Interactions (3)