DasGupta, R., Nybakken, K., Booker, M., Mathey-Prevot, B., Gonsalves, F., Changkakoty, B., Perrimon, N. (2007). A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. Genome Biol. 8(9): R203.
FlyBase ID
FBrf0205348
Publication Type
Research paper
Abstract
Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wingless and Hedgehog signaling pathways using second generation double-stranded RNA libraries. Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes.
