Isolation and characterization of Df(3L)BSC577
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC577 was isolated as a FLP recombinase-induced recombination event involving P{XP}Mob2d11543 and PBac{RB}CG32085e02816. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C,Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}Mob2d11543/PBac{RB}CG32085e02816 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC577 from the segment of P{XP}Mob2d11543 to the left of its FRT site and the segment of PBac{RB}CG32085e02816 to the right of its FRT site. The cytological breakpoints of Df(3L)BSC577 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 68C13;68D1. Df(3L)BSC577 failed to complement rt2 and rtP.