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Cook, K., Christensen, S., Cook, K. (2008.9.3). Isolation and characterization of Df(1)BSC586. 
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FBrf0205899
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From: 	Kevin Cook <kcook@XXXX>
To: 	FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>, kaufmanXXXX
Subject: 	Isolation and characterization of Df(1)BSC586
Date: 	Wed, 03 Sep 2008  11:58:40  -0400  ( 16:58  BST)
Isolation and characterization of Df(1)BSC586
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC586 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d06045 and PBac{WH}CG11710[f03997]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{XP}d06045/PBac{WH}CG11710[f03997]; MKRS, P{hsFLP}86E/+ females 
crossed to Binsinscy/Y males. These females were heat shocked as 
larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC586 from the segment of P{XP}d06045 
to the left of its FRT site and the segment of 
PBac{WH}CG11710[f03997] to the right of its FRT site. Its presence 
was verified using the PCR methods and primers described in Parks et 
al. The cytological breakpoints of Df(1)BSC586 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 18F2;19D1. It failed to complement sw[1].
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    English
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    Aberrations (1)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)