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Citation
Temme, C., Weissbach, R., Lilie, H., Wilson, C., Meinhart, A., Meyer, S., Golbik, R., Schierhorn, A., Wahle, E. (2009). The Drosophila melanogaster Gene cg4930 Encodes a High Affinity Inhibitor for Endonuclease G.  J. Biol. Chem. 284(13): 8337--8348.
FlyBase ID
FBrf0207466
Publication Type
Research paper
Abstract

Endonuclease G (EndoG) is a mitochondrial enzyme believed to be released during apoptosis to participate in the degradation of nuclear DNA. This paper describes a Drosophila protein, EndoGI, which inhibits EndoG specifically. EndoG and EndoGI associate with subpicomolar affinity, forming a 2:1 complex in which dimeric EndoG is bound by two tandemly repeated homologous domains of monomeric EndoGI. Binding appears to involve the active site of EndoG. EndoGI is present in the cell nucleus at micromolar concentrations. Upon induction of apoptosis, levels of the inhibitor appear to be reduced, and it is relocalized to the cytoplasm. EndoGI, encoded by the predicted open reading frame cg4930, is expressed throughout Drosophila development. Flies homozygous for a hypomorphic EndoGI mutation have a strongly reduced viability, which is modulated by genetic background and diet. We propose that EndoGI protects the cell against low levels of EndoG outside mitochondria.

PubMed ID
PubMed Central ID
PMC2659192 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Biol. Chem.
    Title
    Journal of Biological Chemistry
    Publication Year
    1905-
    ISBN/ISSN
    0021-9258
    Data From Reference
    Aberrations (2)
    Alleles (1)
    Genes (7)
    Physical Interactions (4)
    Insertions (1)