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Górska-Andrzejak, J., Salvaterra, P.M., Meinertzhagen, I.A., Krzeptowski, W., Görlich, A., Pyza, E. (2009). Cyclical expression of Na+/K+-ATPase in the visual system of Drosophila melanogaster.  J. Insect Physiol. 55(5): 459--468.
FlyBase ID
FBrf0208075
Publication Type
Research paper
Abstract

In the first (lamina) and second (medulla) optic neuropils of Drosophila melanogaster, sodium pump subunit expression changes during the day and night, controlled by a circadian clock. We examined alpha-subunit expression from the intensity of immunolabeling. For the beta-subunit, encoded by Nervana 2 (Nrv2), we used Nrv2-GAL4 to drive expression of GFP, and measured the resultant fluorescence in whole heads and specific optic lobe cells. All optic neuropils express the alpha-subunit, highest at the beginning of night in both lamina and medulla in day/night condition and the oscillation was maintained in constant darkness. This rhythm was lacking in the clock arrhythmic per(0) mutant. GFP driven by Nrv2 was mostly detected in glial cells, mainly in the medulla. There, GFP expression occurs in medulla neuropil glia (MNGl), which express the clock gene per, and which closely contact the terminals of clock neurons immunoreactive to pigment dispersing factor. GFP fluorescence exhibited circadian oscillation in whole heads from Nrv2-GAL4+UAS-S65T-GFP flies, although significant GFP oscillations were lacking in MNGl, as they were for both subunit mRNAs in whole-head homogenates. In the dissected brain tissues, however, the mRNA of the alpha-subunit showed a robust daily rhythm in concentration changes while changes in the beta-subunit mRNA were weaker and not statistically significant. Thus in the brain, the genes for the sodium pump subunits, at least the one encoding the alpha-subunit, seem to be clock-controlled and the abundance of their corresponding proteins mirrors daily changes in mRNA, showing cyclical accumulation in cells.

PubMed ID
PubMed Central ID
PMC2721802 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Insect Physiol.
    Title
    Journal of Insect Physiology
    Publication Year
    1957-
    ISBN/ISSN
    0022-1910 1879-1611
    Data From Reference
    Aberrations (1)
    Alleles (4)
    Genes (9)
    Natural transposons (1)
    Transgenic Constructs (3)
    Transcripts (1)