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van Gemert, A.M., van der Laan, A.M., Pilgram, G.S., Fradkin, L.G., Noordermeer, J.N., Tanke, H.J., Jost, C.R. (2009). In vivo monitoring of mRNA movement in Drosophila body wall muscle cells reveals the presence of myofiber domains.  PLoS ONE 4(8): e6663.
FlyBase ID
FBrf0208671
Publication Type
Research paper
Abstract

In skeletal muscle each muscle cell, commonly called myofiber, is actually a large syncytium containing numerous nuclei. Experiments in fixed myofibers show that mRNAs remain localized around the nuclei in which they are produced.In this study we generated transgenic flies that allowed us to investigate the movement of mRNAs in body wall myofibers of living Drosophila embryos. We determined the dynamic properties of GFP-tagged mRNAs using in vivo confocal imaging and photobleaching techniques and found that the GFP-tagged mRNAs are not free to move throughout myofibers. The restricted movement indicated that body wall myofibers consist of three domains. The exchange of mRNAs between the domains is relatively slow, but the GFP-tagged mRNAs move rapidly within these domains. One domain is located at the centre of the cell and is surrounded by nuclei while the other two domains are located at either end of the fiber. To move between these domains mRNAs have to travel past centrally located nuclei.These data suggest that the domains made visible in our experiments result from prolonged interactions with as yet undefined structures close to the nuclei that prevent GFP-tagged mRNAs from rapidly moving between the domains. This could be of significant importance for the treatment of myopathies using regenerative cell-based therapies.

PubMed ID
PubMed Central ID
PMC2722729 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    PLoS ONE
    Title
    PLoS ONE
    Publication Year
    2006-
    ISBN/ISSN
    1932-6203
    Data From Reference
    Alleles (5)
    Genes (5)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (6)
    Transgenic Constructs (4)