Weng, R., Chen, Y.W., Bushati, N., Cliffe, A., Cohen, S.M. (2009). Recombinase-mediated cassette exchange provides a versatile platform for gene targeting: knockout of miR-31b. Genetics 183(1): 399--402.
FlyBase ID
FBrf0208945
Publication Type
Research paper
Abstract
A series of vectors has been designed to enhance the versatility of targeted homologous recombination. Recombinase-mediated cassette exchange permits sequential targeting at any locus and improves flexibility in making user-defined mutations. Application of RMCE to delete an intronic microRNA gene is described.
