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Reference
Citation
Lau, N.C., Robine, N., Martin, R., Chung, W.J., Niki, Y., Berezikov, E., Lai, E.C. (2009). Abundant primary piRNAs, endo-siRNAs, and microRNAs in a Drosophila ovary cell line.  Genome Res. 19(10): 1776--1785.
FlyBase ID
FBrf0209029
Publication Type
Research paper
Abstract

Piwi proteins, a subclass of Argonaute-family proteins, carry approximately 24-30-nt Piwi-interacting RNAs (piRNAs) that mediate gonadal defense against transposable elements (TEs). We analyzed the Drosophila ovary somatic sheet (OSS) cell line and found that it expresses miRNAs, endogenous small interfering RNAs (endo-siRNAs), and piRNAs in abundance. In contrast to intact gonads, which contain mixtures of germline and somatic cell types that express different Piwi-class proteins, OSS cells are a homogenous somatic cell population that expresses only PIWI and primary piRNAs. Detailed examination of its TE-derived piRNAs and endo-siRNAs revealed aspects of TE defense that do not rely upon ping-pong amplification. In particular, we provide evidence that a subset of piRNA master clusters, including flamenco, are specifically expressed in OSS and ovarian follicle cells. These data indicate that the restriction of certain TEs in somatic gonadal cells is largely mediated by a primary piRNA pathway.

PubMed ID
PubMed Central ID
PMC2765285 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Lai lab miRNA expression and conservation data for Drosophila.
Mohammed and Lai, 2016.2.8, Lai lab miRNA expression and conservation data for Drosophila. [FBrf0230987]

Associated Information
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Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genome Res.
    Title
    Genome Research
    Publication Year
    1995-
    ISBN/ISSN
    1088-9051
    Data From Reference
    Genes (18)
    Cell Lines (1)
    Natural transposons (15)