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Citation
Okamura, K., Liu, N., Lai, E.C. (2009). Distinct mechanisms for microRNA strand selection by Drosophila Argonautes.  Mol. Cell 36(3): 431--444.
FlyBase ID
FBrf0209322
Publication Type
Research paper
Abstract

In Drosophila, miRNA strands are predominantly sorted into AGO1 to regulate seed-matched target transcripts, while their partner miRNA* strands are thought to be mostly degraded. Here, we report that Drosophila Argonautes exhibit different strand preferences for miRNA duplexes, and that in particular, many miRNA* species accumulate in the RNAi effector AGO2. AGO2-loaded miRNA* species require canonical RNAi factors for their accumulation, are efficiently 3' modified, and are preferentially active on extensively matched target transcripts. Differential miRNA/miRNA* sorting profiles are correlated with specific central mismatches. In vitro assays revealed an active role for Watson-Crick base-pairing at positions 9 and 10 in promoting strand selection by AGO2, with little reciprocal effect on strand selection by AGO1. We conclude that miRNA strand selection and sorting are actually linked processes that stem from distinct loading preferences of AGO proteins and that independent sorting of duplex strands is a general feature of Drosophila microRNA genes.

PubMed ID
PubMed Central ID
PMC2785079 (PMC) (EuropePMC)
Related Publication(s)
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Lai lab miRNA expression and conservation data for Drosophila.
Mohammed and Lai, 2016.2.8, Lai lab miRNA expression and conservation data for Drosophila. [FBrf0230987]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Cell
    Title
    Molecular Cell
    Publication Year
    1997-
    ISBN/ISSN
    1097-2765 1097-4164
    Data From Reference