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Citation
Kalverda, B., Pickersgill, H., Shloma, V.V., Fornerod, M. (2010). Nucleoporins Directly Stimulate Expression of Developmental and Cell-Cycle Genes Inside the Nucleoplasm.  Cell 140(3): 360--371.
FlyBase ID
FBrf0209913
Publication Type
Research paper
Abstract

Nuclear pore complexes (NPCs) mediate transport across the nuclear envelope. In yeast, they also interact with active genes, attracting or retaining them at the nuclear periphery. In higher eukaryotes, some NPC components (nucleoporins) are also found in the nucleoplasm, with a so far unknown function. We have functionally characterized nucleoporin-chromatin interactions specifically at the NPC or within the nucleoplasm in Drosophila. We analyzed genomic interactions of full-length nucleoporins Nup98, Nup50, and Nup62 and nucleoplasmic and NPC-tethered forms of Nup98. We found that nucleoporins predominantly interacted with transcriptionally active genes inside the nucleoplasm, in particular those involved in developmental regulation and the cell cycle. A smaller set of nonactive genes interacted with the NPC. Genes strongly interacting with nucleoplasmic Nup98 were downregulated upon Nup98 depletion and activated on nucleoplasmic Nup98 overexpression. Thus, nucleoporins stimulate developmental and cell-cycle gene expression away from the NPC by interacting with these genes inside the nucleoplasm.

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Obtained with permission from Cell Press.
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PubMed Central ID
Related Publication(s)
Note

Characterization of genome-nucleoporin interactions in Drosophila links chromatin insulators to the nuclear pore complex.
Kalverda and Fornerod, 2010, Cell Cycle 9(24): 4812--4817 [FBrf0212825]

Nups Take Leave of the Nuclear Envelope to Regulate Transcription.
Hou and Corces, 2010, Cell 140(3): 306--308 [FBrf0209899]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference
    Genes (4)
    Cell Lines (1)