Open Close
Reference
Citation
Cook, K., Christensen, S., Cook, K. (2010.2.17). Isolation and characterization of Df(3R)BSC874. 
FlyBase ID
FBrf0210085
Publication Type
Personal communication to FlyBase
Abstract
PubMed ID
PubMed Central ID
Text of Personal Communication
Isolation and characterization of Df(3R)BSC874
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC874 was isolated as a FLP recombinase-induced recombination event involving P{XP}d01701 and PBac{WH}CG14509f04904. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d01701/PBac{WH}CG14509f04904 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC874 from the segment of P{XP}d01701 to the left of its FRT site and the segment of PBac{WH}CG14509f04904 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d01701 to be Release 3 genomic coordinate 24490117 on chromosome arm 3R, which corresponds to Release 5 coordinate  3R:24500751 . The predicted cytology of both coordinates is 98E1. PBac{WH}CG14509f04904 maps to Release 5 coordinate  3R:25017393  with predicted cytology 99A1. Consequently, the breakpoints of Df(3R)BSC874 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3R:24500751 ;25017393 and the cytological breakpoints predicted from these coordinates are 98E1;99A1. Df(3R)BSC874 failed to complement Df(3R)BSC789 and Df(3R)BSC806.
DOI
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Abbreviation
    Title
    ISBN/ISSN
    Data From Reference
    Aberrations (3)
    Insertions (3)
    Transgenic Constructs (1)