Isolation and characterization of Df(2R)BSC889
Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC889 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG11163d05998 and PBac{WH}CG11211f02744. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}CG11163d05998/PBac{WH}CG11211f02744 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC889 from the segment of P{XP}CG11163d05998 to the left of its FRT site and the segment of PBac{WH}CG11211f02744 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}CG11211f02744 to be Release 3 genomic coordinate  2R:1320210 . This corresponds to  2R:2069250  on the Release 5 genome map. The breakpoints of Df(2R)BSC889 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2R:1669744--1669934 ;2069250 and the cytological breakpoints predicted from these coordinates are 41F11;42A13. Df(2R)BSC889 failed to complement EcRM554fs and Df(2R)BSC313.