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Wang, H., Brust-Mascher, I., Cheerambathur, D., Scholey, J.M. (2010). Coupling between microtubule sliding, plus-end growth and spindle length revealed by kinesin-8 depletion.  Cytoskeleton (Hoboken) 67(11): 715--728.
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Research paper

Mitotic spindle length control requires coordination between microtubule (MT) dynamics and motor-generated forces. To investigate how MT plus-end polymerization contributes to spindle length in Drosophila embryos, we studied the dynamics of the MT plus-end depolymerase, kinesin-8, and the effects of kinesin-8 inhibition using mutants and antibody microinjection. As expected, kinesin-8 was found to contribute to anaphase A. Furthermore, kinesin-8 depletion caused: (i) excessive polymerization of interpolar (ip) MT plus ends, which "overgrow" to penetrate distal half spindles; (ii) an increase in the poleward ipMT sliding rate that is coupled to MT plus-end polymerization; (iii) premature spindle elongation during metaphase/anaphase A; and (iv) an increase in the anaphase B spindle elongation rate which correlates linearly with the MT sliding rate. This is best explained by a revised "ipMT sliding/minus-end depolymerization" model for spindle length control which incorporates a coupling between ipMT plus end dynamics and the outward ipMT sliding that drives poleward flux and spindle elongation.

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PMC2998535 (PMC) (EuropePMC)
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    Cytoskeleton (Hoboken)
    Cytoskeleton (Hoboken)
    1949-3584 1949-3592
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    Aberrations (1)
    Alleles (7)
    Genes (7)
    Natural transposons (1)
    Insertions (2)
    Experimental Tools (2)
    Transgenic Constructs (6)