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Neumüller, R.A., Richter, C., Fischer, A., Novatchkova, M., Neumüller, K.G., Knoblich, J.A. (2011). Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi.  Cell Stem Cell 8(5): 580--593.
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Research paper

The balance between stem cell self-renewal and differentiation is precisely controlled to ensure tissue homeostasis and prevent tumorigenesis. Here we use genome-wide transgenic RNAi to identify 620 genes potentially involved in controlling this balance in Drosophila neuroblasts. We quantify all phenotypes and derive measurements for proliferation, lineage, cell size, and cell shape. We identify a set of transcriptional regulators essential for self-renewal and use hierarchical clustering and integration with interaction data to create functional networks for the control of neuroblast self-renewal and differentiation. Our data identify key roles for the chromatin remodeling Brm complex, the spliceosome, and the TRiC/CCT-complex and show that the alternatively spliced transcription factor Lola and the transcriptional elongation factors Ssrp and Barc control self-renewal in neuroblast lineages. As our data are strongly enriched for genes highly expressed in murine neural stem cells, they are likely to provide valuable insights into mammalian stem cell biology as well.

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PubMed Central ID
PMC3093620 (PMC) (EuropePMC)
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    Publication Type
    Cell Stem Cell
    Cell Stem Cell
    Publication Year
    1934-5909 1875-9777
    Data From Reference
    Alleles (19)
    Genes (295)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (2)
    Transgenic Constructs (18)