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Oberegelsbacher, C., Schneidler, C., Voolstra, O., Cerny, A., Huber, A. (2011). The Drosophila TRPL ion channel shares a Rab-dependent translocation pathway with rhodopsin.  Europ. J. Cell Biol. 90(8): 620--630.
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FBrf0213847
Publication Type
Research paper
Abstract

The Drosophila visual transduction cascade is embedded in the rhabdomeres of photoreceptor cells and culminates in the opening of the two ion channels, TRP and TRPL. TRPL translocates from the rhabdomeres to the cell body upon illumination and vice versa when flies are kept in the dark. Here, we studied the mechanisms underlying the light-dependent internalization of TRPL. Co-localization of TRPL and rhodopsin in endocytic particles revealed that TRPL is internalized by a vesicular transport pathway that is also utilized, at least partially, for rhodopsin endocytosis. TRPL internalization is attenuated under light conditions that result in a high rate of rhodopsin internalization and is highest in orange light that result in very little rhodopsin internalization. In line with a canonical vesicular transport pathway, we found that rab proteins, Rab5 and RabX4, are required for the internalization of TRPL into the cell body. Our results provide insight into stimulus-dependent internalization of a prominent member of the TRP superfamily.

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    Language of Publication
    English
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    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Europ. J. Cell Biol.
    Title
    European Journal of Cell Biology
    Publication Year
    1979-
    ISBN/ISSN
    0171-9335
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