Open Close
Reference
Citation
Smart, A.D., Course, M.M., Rawson, J., Selleck, S., Van Vactor, D., Johnson, K.G. (2011). Heparan sulfate proteoglycan specificity during axon pathway formation in the Drosophila embryo.  Dev. Neurobiol. 71(7): 608--618.
FlyBase ID
FBrf0213870
Publication Type
Research paper
Abstract

Axon guidance is influenced by the presence of heparan sulfate (HS) proteoglycans (HSPGs) on the surface of axons and growth cones (Hu, [2001]: Nat Neurosci 4:695-701 ; Irie et al. [2002]: Development 129:61-70 ; Inatani et al. [2003]: Science 302:1044-1046 ; Johnson et al. [2004]: Curr Biol 14:499-504 ; Steigemann et al. [2004]: Curr Biol 14:225-230 ). Multiple HSPGs, including Syndecans, Glypicans and Perlecans, carry the same carbohydrate polymer backbones, raising the question of how these molecules display functional specificity during nervous system development. Here we use the Drosophila central nervous system (CNS) as a model to compare the impact of eliminating Syndecan (Sdc) and/or the Glypican Dally-like (Dlp). We show that Dlp and Sdc share a role in promoting accurate patterns of axon fasciculation in the lateral longitudinal neuropil; however, unlike mutations in sdc, which disrupt the ability of the secreted repellent Slit to prevent inappropriate passage of axons across the midline, mutations in dlp show neither midline defects nor genetic interactions with Slit and its Roundabout (Robo) receptors at the midline. Dlp mutants do show genetic interactions with Slit and Robo in lateral fascicle formation. In addition, simultaneous loss of Dlp and Sdc demonstrates an important role for Dlp in midline repulsion, reminiscent of the functional overlap between Robo receptors. A comparison of HSPG distribution reveals a pattern that leaves midline proximal axons with relatively little Dlp. Finally, the loss of Dlp alters Slit distribution distal but not proximal to the midline, suggesting that distinct yet overlapping pattern of HSPG expression provides a spatial system that regulates axon guidance decisions.

PubMed ID
PubMed Central ID
PMC3115403 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Neurobiol.
    Title
    Developmental Neurobiology
    Publication Year
    2007--
    ISBN/ISSN
    1932-8451 1932-846X
    Data From Reference
    Genes (6)