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Citation
Zhong, L., Bellemer, A., Yan, H., Honjo, K., Robertson, J., Hwang, R.Y., Pitt, G.S., Tracey, W.D. (2012). Thermosensory and non-thermosensory isoforms of Drosophila melanogaster TRPA1 reveal heat sensor domains of a thermoTRP channel.  Cell Rep. 1(1): 43--55.
FlyBase ID
FBrf0217493
Publication Type
Research paper
Abstract

Specialized somatosensory neurons detect temperatures ranging from pleasantly cool or warm to burning hot and painful (nociceptive). The precise temperature ranges sensed by thermally sensitive neurons is determined by tissue-specific expression of ion channels of the transient receptor potential(TRP) family.We show here that in Drosophila, TRPA1 is required for the sensing of nociceptive heat. We identify two previously unidentified protein isoforms of dTRPA1, named dTRPA1-C and dTRPA1-D, that explain this requirement. A dTRPA1-C/D reporter was exclusively expressed in nociceptors, and dTRPA1-C rescued thermal nociception phenotypes when restored to mutant nociceptors. However,surprisingly, we find that dTRPA1-C is not a direct heat sensor. Alternative splicing generates at least four isoforms of dTRPA1. Our analysis of these isoforms reveals a 37-amino-acid-long intracellular region (encoded by a single exon) that is critical for dTRPA1 temperature responses. The identification of these amino acids opens the door to a biophysical understanding of a molecular thermosensor.

Graphical Abstract
Obtained with permission from Cell Press.
PubMed ID
PubMed Central ID
PMC3278078 (PMC) (EuropePMC)
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Secondary IDs
  • FBrf0217572
Language of Publication
English
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Parent Publication
Publication Type
Journal
Abbreviation
Cell Rep.
Title
Cell reports
ISBN/ISSN
2211-1247
Data From Reference
Aberrations (1)
Alleles (10)
Genes (4)
Sequence Features (1)
Cell Lines (1)
Natural transposons (1)
Experimental Tools (2)
Transgenic Constructs (9)
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