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Citation
Paik, D., Jang, Y.G., Lee, Y.E., Lee, Y.N., Yamamoto, R., Gee, H.Y., Yoo, S., Bae, E., Min, K.J., Tatar, M., Park, J.J. (2012). Misexpression screen delineates novel genes controlling Drosophila lifespan.  Mech. Ageing Dev. 133(5): 234--245.
FlyBase ID
FBrf0218389
Publication Type
Research paper
Abstract

In an initial preliminary screen we identified factors associated with controlling Drosophila aging by examining longevity in adults where EP elements induced over-expression or antisense-RNA at genes adjacent to each insertion. Here, we study 45 EP lines that initially showed at least 10% longer mean lifespan than controls. These 45 lines and a daughterless (da)-Gal4 stock were isogenized into a CS10 wild-type background. Sixteen EP lines corresponding to 15 genes significantly extended lifespan when their target genes were driven by da-Gal4. In each case, the target genes were seen to be over-expressed. Independently derived UAS-gene transgenic stocks were available or made for two candidates: ImpL2 which is ecdysone-inducible gene L2, and CG33138, 1,4-alpha-glucan branching enzyme. With both, adult lifespan was increased upon over-expression via the GeneSwitch inducible Gal4 driver system. Several genes in this set of 15 correspond to previously discovered longevity assurance systems such as insulin/IGF-1 signaling, gene silencing, and autophagy; others suggest new potential mechanisms for the control of aging including mRNA synthesis and maturation, intracellular vesicle trafficking, and neuroendocrine regulation.

PubMed ID
PubMed Central ID
PMC3359393 (PMC) (EuropePMC)
Associated Information
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mech. Ageing Dev.
    Title
    Mechanisms of Ageing and Development
    Publication Year
    1972-
    ISBN/ISSN
    0047-6374
    Data From Reference
    Alleles (52)
    Genes (53)
    Natural transposons (1)
    Insertions (46)
    Experimental Tools (1)
    Transgenic Constructs (7)
    Transcripts (1)