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Citation
Siddiqui, N.U., Li, X., Luo, H., Karaiskakis, A., Hou, H., Kislinger, T., Westwood, J.T., Morris, Q., Lipshitz, H.D. (2012). Genome-wide analysis of the maternal-to-zygotic transition in Drosophila primordial germ cells.  Genome Biol. 13(2): R11.
FlyBase ID
FBrf0218847
Publication Type
Research paper
Abstract

During the maternal-to-zygotic transition (MZT) vast changes in the embryonic transcriptome are produced by a combination of two processes: elimination of maternally provided mRNAs and synthesis of new transcripts from the zygotic genome. Previous genome-wide analyses of the MZT have been restricted to whole embryos. Here we report the first such analysis for primordial germ cells (PGCs), the progenitors of the germ-line stem cells.We purified PGCs from Drosophila embryos, defined their proteome and transcriptome, and assessed the content, scale and dynamics of their MZT. Transcripts encoding proteins that implement particular types of biological functions group into nine distinct expression profiles, reflecting coordinate control at the transcriptional and posttranscriptional levels. mRNAs encoding germ-plasm components and cell-cell signaling molecules are rapidly degraded while new transcription produces mRNAs encoding the core transcriptional and protein synthetic machineries. The RNA-binding protein Smaug is essential for the PGC MZT, clearing transcripts encoding proteins that regulate stem cell behavior, transcriptional and posttranscriptional processes. Computational analyses suggest that Smaug and AU-rich element binding proteins function independently to control transcript elimination.The scale of the MZT is similar in the soma and PGCs. However, the timing and content of their MZTs differ, reflecting the distinct developmental imperatives of these cell types. The PGC MZT is delayed relative to that in the soma, likely because relief of PGC-specific transcriptional silencing is required for zygotic genome activation as well as for efficient maternal transcript clearance.

PubMed ID
PubMed Central ID
PMC3334568 (PMC) (EuropePMC)
Related Publication(s)
Note

Maternal-to-zygotic transition: soma versus germline.
Simonelig, 2012, Genome Biol. 13(2): 145 [FBrf0218830]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genome Biol.
    Title
    Genome Biology
    Publication Year
    2000-
    ISBN/ISSN
    1474-7596 1474-760X
    Data From Reference