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Citation
Kaushik, G., Zambon, A.C., Fuhrmann, A., Bernstein, S.I., Bodmer, R., Engler, A.J., Cammarato, A., Mercola, M. (2012). Measuring passive myocardial stiffness in Drosophila melanogaster to investigate diastolic dysfunction.  J. Cell. Mol. Med. 16(8): 1656--1662.
FlyBase ID
FBrf0219008
Publication Type
Review
Abstract

Aging is marked by a decline in LV diastolic function, which encompasses abnormalities in diastolic relaxation, chamber filling and/or passive myocardial stiffness. Genetic tractability and short life span make Drosophila melanogaster an ideal organism to study the effects of aging on heart function, including senescent-associated changes in gene expression and in passive myocardial stiffness. However, use of the Drosophila heart tube to probe deterioration of diastolic performance is subject to at least two challenges: the extent of genetic homology to mammals and the ability to resolve mechanical properties of the bilayered fly heart, which consists of a ventral muscle layer that covers the contractile cardiomyocytes. Here, we argue for widespread use of Drosophila as a novel myocardial aging model by (1) describing diastolic dysfunction in flies, (2) discussing how critical pathways involved in dysfunction are conserved across species and (3) demonstrating the advantage of an atomic force microscopy-based analysis method to measure stiffness of the multilayered Drosophila heart tube versus isolated myocytes from other model systems. By using powerful Drosophila genetic tools, we aim to efficiently alter changes observed in factors that contribute to diastolic dysfunction to understand how one might improve diastolic performance at advanced ages in humans.

PubMed ID
PubMed Central ID
PMC3326184 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell. Mol. Med.
    Title
    Journal of cellular and molecular medicine
    Publication Year
    2000-
    ISBN/ISSN
    1582-1838 1582-4934
    Data From Reference
    Genes (28)
    Human Disease Models (1)