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Laneve, P., Delaporte, C., Trebuchet, G., Komonyi, O., Flici, H., Popkova, A., D'Agostino, G., Taglini, F., Kerekes, I., Giangrande, A. (2013). The Gcm/Glide molecular and cellular pathway: New actors and new lineages.  Dev. Biol. 375(1): 65--78.
FlyBase ID
FBrf0220777
Publication Type
Research paper
Abstract

In Drosophila, the transcription factor Gcm/Glide plays a key role in cell fate determination and cellular differentiation. In light of its crucial biological impact, major efforts have been put for analyzing its properties as master regulator, from both structural and functional points of view. However, the lack of efficient antibodies specific to the Gcm/Glide protein precluded thorough analyses of its regulation and activity in vivo. In order to relieve such restraints, we designed an epitope-tagging approach to "FLAG"-recognize and analyze the functional protein both in vitro (exogenous Gcm/Glide) and in vivo (endogenous protein). We here (i) reveal a tight interconnection between the small RNA and the Gcm/Glide pathways. AGO1 and miR-1 are Gcm/Glide targets whereas miR-279 negatively controls Gcm/Glide expression (ii) identify a novel cell population, peritracheal cells, expressing and requiring Gcm/Glide. Peritracheal cells are non-neuronal neurosecretory cells that are essential in ecdysis. In addition to emphasizing the importance of following the distribution and the activity of endogenous proteins in vivo, this study provides new insights and a novel frame to understand the Gcm/Glide biology.

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Biol.
    Title
    Developmental Biology
    Publication Year
    1959-
    ISBN/ISSN
    0012-1606
    Data From Reference
    Aberrations (1)
    Alleles (17)
    Genes (11)
    Physical Interactions (4)
    Polypeptides (1)
    Cell Lines (1)
    Natural transposons (2)
    Insertions (2)
    Experimental Tools (3)
    Transgenic Constructs (10)
    Transcripts (2)