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Almudi, I., Poernbacher, I., Hafen, E., Stocker, H. (2013). The Lnk/SH2B adaptor provides a fail-safe mechanism to establish the Insulin receptor-Chico interaction.  Cell Commun. Signal. 11(1): 26.
FlyBase ID
FBrf0221426
Publication Type
Research paper
Abstract

Insulin/insulin-like growth factor signalling (IIS) has been described as one of the major pathways involved in growth control and homeostasis in multicellular organisms. Whereas its core components are well established, less is known about the molecular functions of IIS regulators. The adaptor molecule Lnk/SH2B has been implicated in IIS but the mechanism by which it promotes IIS activity has remained enigmatic.In this study, we analyse genetic and physical interactions among InR, Chico and Lnk in Drosophila tissues. FRET analysis reveals in vivo binding between all three molecules. Genetically, Lnk acts upstream of Chico. We demonstrate that Chico's plasma membrane localisation is ensured by both its PH domain and by the interaction with Lnk. Furthermore, Lnk is able to recruit an intracellular InR fragment to the membrane.Thus, by acting as a scaffolding molecule that ensures InR and Chico enrichment at the membrane, Lnk provides a fail-safe mechanism for IIS activation.

PubMed ID
PubMed Central ID
PMC3637499 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell Commun. Signal.
    Title
    Cell communication and signaling : CCS
    ISBN/ISSN
    1478-811X
    Data From Reference
    Alleles (10)
    Gene Groups (1)
    Genes (5)
    Physical Interactions (3)
    Natural transposons (1)
    Experimental Tools (3)
    Transgenic Constructs (8)