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Citation
Schaaf, C.A., Misulovin, Z., Gause, M., Koenig, A., Gohara, D.W., Watson, A., Dorsett, D. (2013). Cohesin and polycomb proteins functionally interact to control transcription at silenced and active genes.  PLoS Genet. 9(6): e1003560.
FlyBase ID
FBrf0221996
Publication Type
Research paper
Abstract

Cohesin is crucial for proper chromosome segregation but also regulates gene transcription and organism development by poorly understood mechanisms. Using genome-wide assays in Drosophila developing wings and cultured cells, we find that cohesin functionally interacts with Polycomb group (PcG) silencing proteins at both silenced and active genes. Cohesin unexpectedly facilitates binding of Polycomb Repressive Complex 1 (PRC1) to many active genes, but their binding is mutually antagonistic at silenced genes. PRC1 depletion decreases phosphorylated RNA polymerase II and mRNA at many active genes but increases them at silenced genes. Depletion of cohesin reduces long-range interactions between Polycomb Response Elements in the invected-engrailed gene complex where it represses transcription. These studies reveal a previously unrecognized role for PRC1 in facilitating productive gene transcription and provide new insights into how cohesin and PRC1 control development.

PubMed ID
PubMed Central ID
PMC3688520 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    PLoS Genet.
    Title
    PLoS Genetics
    Publication Year
    2005-
    ISBN/ISSN
    1553-7404 1553-7390
    Data From Reference
    Alleles (15)
    Genes (19)
    Cell Lines (2)
    Insertions (1)
    Transgenic Constructs (2)