Open Close
Reference
Citation
Zhang, Q., Shalaby, N.A., Buszczak, M. (2014). Changes in rRNA transcription influence proliferation and cell fate within a stem cell lineage.  Science 343(6168): 298--301.
FlyBase ID
FBrf0223821
Publication Type
Research paper
Abstract

Ribosome biogenesis drives cell growth and proliferation, but mechanisms that modulate this process within specific lineages remain poorly understood. Here, we identify a Drosophila RNA polymerase I (Pol I) regulatory complex composed of Under-developed (Udd), TAF1B, and a TAF1C-like factor. Disruption of udd or TAF1B results in reduced ovarian germline stem cell (GSC) proliferation. Female GSCs display high levels of ribosomal RNA (rRNA) transcription, and Udd becomes enriched in GSCs relative to their differentiating daughters. Increasing Pol I transcription delays differentiation, whereas reducing rRNA production induces both morphological changes that accompany multicellular cyst formation and specific decreased expression of the bone morphogenetic protein (BMP) pathway component Mad. These findings demonstrate that modulating rRNA synthesis fosters changes in the cell fate, growth, and proliferation of female Drosophila GSCs and their daughters.

PubMed ID
PubMed Central ID
PMC4084784 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Science
    Title
    Science
    Publication Year
    1895-
    ISBN/ISSN
    0036-8075
    Data From Reference
    Aberrations (1)
    Alleles (11)
    Genes (16)
    Physical Interactions (18)
    Cell Lines (1)
    Natural transposons (2)
    Insertions (4)
    Experimental Tools (3)
    Transgenic Constructs (6)