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Kongton, K., McCall, K., Phongdara, A. (2014). Identification of gamma-interferon-inducible lysosomal thiol reductase (GILT) homologues in the fruit fly Drosophila melanogaster.  Dev. Comp. Immunol. 44(2): 389--396.
FlyBase ID
FBrf0224365
Publication Type
Research paper
Abstract

Gamma-interferon-inducible lysosomal thiol reductase (GILT) has been demonstrated to be involved in the immune response to bacterial challenge in various organisms. However, little is known about GILT function in innate immunity. Drosophila has been commonly used as a model for the study of the innate immune response of invertebrates. Here, we identify the CG9796, CG10157, and CG13822 genes of fruit fly Drosophila melanogaster as GILT homologues. All deduced Drosophila GILT coding sequences contained the major characteristic features of the GILT protein family: the GILT signature CQHGX2ECX2NX4C sequence and the active site CXXC or CXXS motif. The mRNA transcript levels of the Drosophila GILT genes were up-regulated after Gram-negative bacteria Escherichia coli DH5α infection. Moreover, a bacterial load assay showed that over-expression of Drosophila GILT in fat body or hemocytes led to a low bacterial colony number whereas knock-down of Drosophila GILT in fat body or hemocytes led to a high bacterial colony number when compared to a wild-type control. These results indicate that the Drosophila GILTs are very likely to play a role in the innate immune response upon bacterial challenge of Drosophila host defense. This study may provide the basis for further study on GILT function in innate immunity.

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PubMed Central ID
Related Publication(s)
Personal communication to FlyBase

GILT genes in D. melanogaster.
Phongdara, 2014.5.22, GILT genes in D. melanogaster. [FBrf0225154]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Comp. Immunol.
    Title
    Developmental and Comparative Immunology
    Publication Year
    1977-
    ISBN/ISSN
    0145-305X
    Data From Reference
    Alleles (9)
    Genes (5)
    Human Disease Models (1)
    Insertions (3)
    Transgenic Constructs (6)