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Citation
Gaspar, I., Yu, Y.V., Cotton, S.L., Kim, D.H., Ephrussi, A., Welte, M.A. (2014). Klar ensures thermal robustness of oskar localization by restraining RNP motility.  J. Cell Biol. 206(2): 199--215.
FlyBase ID
FBrf0225715
Publication Type
Research paper
Abstract

Communication usually applies feedback loop-based filters and amplifiers to ensure undistorted delivery of messages. Such an amplifier acts during Drosophila melanogaster midoogenesis, when oskar messenger ribonucleic acid (mRNA) anchoring depends on its own locally translated protein product. We find that the motor regulator Klar β mediates a gain-control process that prevents saturation-based distortions in this positive feedback loop. We demonstrate that, like oskar mRNA, Klar β localizes to the posterior pole of oocytes in a kinesin-1-dependent manner. By live imaging and semiquantitative fluorescent in situ hybridization, we show that Klar β restrains oskar ribonucleoprotein motility and decreases the posterior-ward translocation of oskar mRNA, thereby adapting the rate of oskar delivery to the output of the anchoring machinery. This negative regulatory effect of Klar is particularly important for overriding temperature-induced changes in motility. We conclude that by preventing defects in oskar anchoring, this mechanism contributes to the developmental robustness of a poikilothermic organism living in a variable temperature environment.

PubMed ID
PubMed Central ID
PMC4107779 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference
    Aberrations (2)
    Alleles (15)
    Genes (6)
    Physical Interactions (6)
    Natural transposons (1)
    Experimental Tools (3)
    Transgenic Constructs (5)