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Citation
Koyama, T., Rodrigues, M.A., Athanasiadis, A., Shingleton, A.W., Mirth, C.K. (2014). Nutritional control of body size through FoxO-Ultraspiracle mediated ecdysone biosynthesis.  eLife 3(): e03091.
FlyBase ID
FBrf0226858
Publication Type
Research paper
Abstract

Despite their fundamental importance for body size regulation, the mechanisms that stop growth are poorly understood. In Drosophila melanogaster, growth ceases in response to a peak of the molting hormone ecdysone that coincides with a nutrition-dependent checkpoint, critical weight. Previous studies indicate that insulin/insulin-like growth factor signaling (IIS)/Target of Rapamycin (TOR) signaling in the prothoracic glands (PGs) regulates ecdysone biosynthesis and critical weight. Here we elucidate a mechanism through which this occurs. We show that Forkhead Box class O (FoxO), a negative regulator of IIS/TOR, directly interacts with Ultraspiracle (Usp), part of the ecdysone receptor. While overexpressing FoxO in the PGs delays ecdysone biosynthesis and critical weight, disrupting FoxO-Usp binding reduces these delays. Further, feeding ecdysone to larvae eliminates the effects of critical weight. Thus, nutrition controls ecdysone biosynthesis partially via FoxO-Usp prior to critical weight, ensuring that growth only stops once larvae have achieved a target nutritional status.

PubMed ID
PubMed Central ID
PMC4337420 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Aberrations (1)
    Alleles (15)
    Genes (7)
    Physical Interactions (2)
    Natural transposons (2)
    Insertions (4)
    Experimental Tools (2)
    Transgenic Constructs (10)
    Transcripts (1)