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Citation
Wong, C.O., Chen, K., Lin, Y.Q., Chao, Y., Duraine, L., Lu, Z., Yoon, W.H., Sullivan, J.M., Broadhead, G.T., Sumner, C.J., Lloyd, T.E., Macleod, G.T., Bellen, H.J., Venkatachalam, K. (2014). A TRPV Channel in Drosophila Motor Neurons Regulates Presynaptic Resting Ca(2+) Levels, Synapse Growth, and Synaptic Transmission.  Neuron 84(4): 764--777.
FlyBase ID
FBrf0226946
Publication Type
Research paper
Abstract

Presynaptic resting Ca(2+) influences synaptic vesicle (SV) release probability. Here, we report that a TRPV channel, Inactive (Iav), maintains presynaptic resting Ca(2+) by promoting Ca(2+) release from the endoplasmic reticulum in Drosophila motor neurons, and is required for both synapse development and neurotransmission. We find that Iav activates the Ca(2+)/calmodulin-dependent protein phosphatase calcineurin, which is essential for presynaptic microtubule stabilization at the neuromuscular junction. Thus, loss of Iav induces destabilization of presynaptic microtubules, resulting in diminished synaptic growth. Interestingly, expression of human TRPV1 in Iav-deficient motor neurons rescues these defects. We also show that the absence of Iav causes lower SV release probability and diminished synaptic transmission, whereas Iav overexpression elevates these synaptic parameters. Together, our findings indicate that Iav acts as a key regulator of synaptic development and function by influencing presynaptic resting Ca(2+).

PubMed ID
PubMed Central ID
PMC4254599 (PMC) (EuropePMC)
Related Publication(s)
Note

TRPV1 Channels: Not So Inactive on the ER.
Imler and Zinsmaier, 2014, Neuron 84(4): 659--661 [FBrf0227012]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Neuron
    Title
    Neuron
    Publication Year
    1988-
    ISBN/ISSN
    0896-6273
    Data From Reference