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Citation
Martín-Bermudo, M.D., Bardet, P.L., Bellaïche, Y., Malartre, M. (2015). The vav oncogene antagonises EGFR signalling and regulates adherens junction dynamics during Drosophila eye development.  Development 142(8): 1492--1501.
FlyBase ID
FBrf0228035
Publication Type
Research paper
Abstract

Organ shaping and patterning depends on the coordinated regulation of multiple processes. The Drosophila compound eye provides an excellent model to study the coordination of cell fate and cell positioning during morphogenesis. Here, we find that loss of vav oncogene function during eye development is associated with a disorganised retina characterised by the presence of additional cells of all types. We demonstrate that these defects result from two distinct roles of Vav. First, and in contrast to its well-established role as a positive effector of the EGF receptor (EGFR), we show that readouts of the EGFR pathway are upregulated in vav mutant larval eye disc and pupal retina, indicating that Vav antagonises EGFR signalling during eye development. Accordingly, decreasing EGFR signalling in vav mutant eyes restores retinal organisation and rescues most vav mutant phenotypes. Second, using live imaging in the pupal retina, we observe that vav mutant cells do not form stable adherens junctions, causing various defects, such as recruitment of extra primary pigment cells. In agreement with this role in junction dynamics, we observe that these phenotypes can be exacerbated by lowering DE-Cadherin or Cindr levels. Taken together, our findings establish that Vav acts at multiple times during eye development to prevent excessive cell recruitment by limiting EGFR signalling and by regulating junction dynamics to ensure the correct patterning and morphogenesis of the Drosophila eye.

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PubMed Central ID
Related Publication(s)
Personal communication to FlyBase

Location data for Vav deletions.
Malartre, 2017.2.20, Location data for Vav deletions. [FBrf0234862]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Development
    Title
    Development
    Publication Year
    1987-
    ISBN/ISSN
    0950-1991
    Data From Reference
    Alleles (18)
    Gene Groups (1)
    Genes (11)
    Natural transposons (1)
    Transgenic Constructs (5)