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Sajwan, S., Sidorov, R., Stašková, T., Žaloudíková, A., Takasu, Y., Kodrík, D., Zurovec, M. (2015). Targeted mutagenesis and functional analysis of adipokinetic hormone-encoding gene in Drosophila.  Insect Biochem. Mol. Biol. 61(): 79--86.
FlyBase ID
FBrf0228509
Publication Type
Research paper
Abstract

Adipokinetic hormones (Akhs) are small peptides (8-10 amino acid aa residues long) found in insects that regulate metabolic responses to stress by stimulating catabolic reactions and mobilizing energy stores. We employed Transcription activator-like effector nuclease (TALEN) mutagenesis and isolated an Akh(1) mutant carrying a small deletion in the gene that resulted in a truncated peptide; the second aa (Leu) was missing from the functional octapeptide. This null Dmel/Akh mutant is suitable to study Akh function without any effect on the C-terminal associated peptide encoded by the same gene. The mutant flies were fully viable and compared to the control flies, had significantly low levels of hemolymph saccharides including trehalose and were resistant to starvation. These characteristics are similar to those obtained from the flies carrying targeted ablation of Akh-expressing neurons (reported earlier). We also found that the Akh(1) mutants are slightly heavy and had a slow metabolic rate. Furthermore, we showed that the ectopic expression of Dmel∖Akh reverses the Akh(1) phenotype and restores the wild-type characteristics. Our results confirmed that Akh is an important regulator of metabolic homeostasis in Drosophila.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Insect Biochem. Mol. Biol.
    Title
    Insect Biochemistry and Molecular Biology
    Publication Year
    1992-
    ISBN/ISSN
    0965-1748
    Data From Reference
    Alleles (5)
    Genes (3)
    Transgenic Constructs (4)