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Citation
Inaba, M., Buszczak, M., Yamashita, Y.M. (2015). Nanotubes mediate niche-stem-cell signalling in the Drosophila testis.  Nature 523(7560): 329--332.
FlyBase ID
FBrf0229034
Publication Type
Research paper
Abstract

Stem cell niches provide resident stem cells with signals that specify their identity. Niche signals act over a short range such that only stem cells but not their differentiating progeny receive the self-renewing signals. However, the cellular mechanisms that limit niche signalling to stem cells remain poorly understood. Here we show that the Drosophila male germline stem cells form previously unrecognized structures, microtubule-based nanotubes, which extend into the hub, a major niche component. Microtubule-based nanotubes are observed specifically within germline stem cell populations, and require intraflagellar transport proteins for their formation. The bone morphogenetic protein (BMP) receptor Tkv localizes to microtubule-based nanotubes. Perturbation of microtubule-based nanotubes compromises activation of Dpp signalling within germline stem cells, leading to germline stem cell loss. Moreover, Dpp ligand and Tkv receptor interaction is necessary and sufficient for microtubule-based nanotube formation. We propose that microtubule-based nanotubes provide a novel mechanism for selective receptor-ligand interaction, contributing to the short-range nature of niche-stem-cell signalling.

PubMed ID
PubMed Central ID
PMC4586072 (PMC) (EuropePMC)
Related Publication(s)
Note

MT-Nanotubes: Lifelines for Stem Cells.
Amoyel and Bach, 2015, Cell Stem Cell 17(2): 133--134 [FBrf0229207]

Developmental biology: Nanotubes in the niche.
Kornberg and Gilboa, 2015, Nature 523(7560): 292--293 [FBrf0231168]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nature
    Title
    Nature
    Publication Year
    1869-
    ISBN/ISSN
    0028-0836
    Data From Reference
    Alleles (41)
    Genes (21)
    Natural transposons (1)
    Insertions (9)
    Experimental Tools (4)
    Transgenic Constructs (33)