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Citation
Yang, F., Zhao, R., Fang, X., Huang, H., Xuan, Y., Ma, Y., Chen, H., Cai, T., Qi, Y., Xi, R. (2015). The RNA surveillance complex Pelo-Hbs1 is required for transposon silencing in the Drosophila germline.  EMBO Rep. 16(8): 965--974.
FlyBase ID
FBrf0229215
Publication Type
Research paper
Abstract

Silencing of transposable elements (TEs) in the metazoan germline is critical for genome integrity and is primarily dependent on Piwi proteins and associated RNAs, which exert their function through both transcriptional and posttranscriptional mechanisms. Here, we report that the evolutionarily conserved Pelo (Dom34)-Hbs1 mRNA surveillance complex is required for transposon silencing in the Drosophila germline. In pelo mutant gonads, mRNAs and proteins of some selective TEs are up-regulated. Pelo is not required for piRNA biogenesis, and our studies suggest that Pelo may function at the translational level to silence TEs: This function requires interaction with Hbs1, and overexpression of RpS30a partially reverts TE-silencing defects in pelo mutants. Interestingly, TE silencing and spermatogenesis defects in pelo mutants can also effectively be rescued by expressing the mammalian ortholog of Pelo. We propose that the Pelo-Hbs1 surveillance complex provides another level of defense against the expression of TEs in the germline of Drosophila and possibly all metazoa.

PubMed ID
PubMed Central ID
PMC4552489 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Location data for pelo[PB60] deletion.
Xi, 2017.7.11, Location data for pelo[PB60] deletion. [FBrf0236023]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    EMBO Rep.
    Title
    EMBO Reports
    Publication Year
    2000-
    ISBN/ISSN
    1469-221X 1469-3178
    Data From Reference